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Knockdown of CNGB3 suppressed the intracellular Ca 2+ concentration and upregulated the expression of MHC-I in GC cells. (A,B) Intracellular Ca 2+ levels following CNGB3 gene knockdown. Confocal illustration of intracellular Ca 2+ imaging (A) and quantification (B). Scale bar: 100 μm (staining method: Fluo-4 AM staining; magnification: 100×). (C-E) Expression level of MHC-I molecules in cells after CNGB3 gene knockdown. **, P<0.01; ***, P<0.001. GC, gastric cancer; MHC-I, major histocompatibility complex class I.

Journal: Translational Cancer Research

Article Title: Identification of a novel ion channel-related gene signature to predict prognosis and immune response of gastric cancer

doi: 10.21037/tcr-2025-1-2874

Figure Lengend Snippet: Knockdown of CNGB3 suppressed the intracellular Ca 2+ concentration and upregulated the expression of MHC-I in GC cells. (A,B) Intracellular Ca 2+ levels following CNGB3 gene knockdown. Confocal illustration of intracellular Ca 2+ imaging (A) and quantification (B). Scale bar: 100 μm (staining method: Fluo-4 AM staining; magnification: 100×). (C-E) Expression level of MHC-I molecules in cells after CNGB3 gene knockdown. **, P<0.01; ***, P<0.001. GC, gastric cancer; MHC-I, major histocompatibility complex class I.

Article Snippet: Cellular Ca 2+ levels were measured using a Fluo-4 AM Fluorescence Calcium Ion Detection Kit (Servicebio, Wuhan, China).

Techniques: Knockdown, Concentration Assay, Expressing, Imaging, Staining, Immunopeptidomics